The present study was conducted for molecular characterization of Salmonella Serovars that isolated from 200 samples including 100 food samples of animal origin (chicken meat, Chicken liver, Minced meat and meat products) purchased from retail stores , street vendors and supermarkets, 50 hand swab samples from handlers in retail stores or markets and 50 human diarrhaeic stool samples were collected from several hospital located in Alexandria province, Egypt. Samples were examined bacteriologically according to ISO 6579:2002 Al: 2007. Then confirmed by biochemical tests, serotyping, and molecular characterization.
The overall proportion of Salmonella positive was 4% (8/200) represented as 3%, 2%, and 2% from food samples, hand swab and diarrhoeic stool samples, respectively. Salmonella isolates were serologically typed as S. Enteritidis, S. Kentucky, and S. Tennesee. Also, Polymerase chain reaction (PCR) was carried out to detected invA (universal gene) and stn (Enterotoxin genes) for the Salmonella serovars , all Salmonella isolates were positive for (invA), while 87.5% were positive for (stn). Antibiogram profile for all Sallmonella isolates were carried out showing presence of multidrug resistance , all of the 8 Salmonella isolates tested (100%) were resistant to gentamicin, amikacin, cefuroxime and cefuroxime axetil.Also both S. Kentucky and S. Enteritidis (87.5%) isolated from food and human sample were rsistant to ampicillin, titarcyclin and trimethoprim/sulfamethoxazole. Two Salmonella isolates(S. Kentucky and S. Enteritidis) were isolated from human samples (25%) were resistant to ciprofloxacin and moxifloxacin.Only S. Kentucky (12.5%) isolated from human samples were resistant to cefotaxime ,cefodoxime, and intermediate to tigecyclin .
Key words: . Enteritidis, S. Kentucky, S. Tennesee , conventional PCR ,inva gene, stn gene, Antibiogram, Multidrug resistant.
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