Uropathogenic Escherichia coli (UPEC) is a fundamental cause of nosocomial urinary tract infection (UTI). UPEC can form biofilms on the mucosa of the urinary bladder and the surface of urethral catheters, thus causing clinical problems. Therefore, the current study aimed at the potential of synbiotics to manage biofilm-associated UTIs by analyzing biofilm inhibition UPEC with cell-free supernatant (CFS) of synbiotics. Biofilm inhibition was accomplished by inoculating each microbial suspension into 96-well microplates on tryptic soy broth (TSB) medium at 37ºC for 48 hours and using a microtiter plate reader at 595nm to read the OD value. The outcome (%) was calculated from the OD value of CFS treatment with UPEC. The result of this research was that each CFS of synbiotic treatment displayed significantly different (P
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