Home|Journals|Articles by Year|Audio Abstracts
 

Original Research



Prevention of Calcium Oxalate Crystal Formation with Medicinal Mushroom Extract with Antioxidant Activity in a Rat Model

Jonathan Wagmaister, Nikhil Gopal, Neel Patel, Muhammad Choudhury, Majid Eshghi, Sensuke Konno.




Abstract

Background: Oxidative stress (OXS) is believed to play a significant role in the development of nephrolithiasis. This possibility was tested by a medicinal mushroom extract, PE, with antioxidant activity capable of diminishing OXS. We examined whether PE would protect renal cells from OXS in vitro and prevent the calcium oxalate (CaOx) crystal formation in a rat model (in vivo).
Methods: Antioxidant activity of PE was assessed if it could reduce OXS exerted by hydrogen peroxide (H2O2), a typical OXS inducer, in renal epithelial MDCK cells. Whether PE may also prevent/reduce the CaOx crystal formation, which was chemically induced by orally giving the rats ethylene glycol (EG), was examined.
Results: The reduction in cell viability with elevated OXS by H2O2 was significantly prevented with PE in MDCK cells. Such elevated OXS was also diminished with PE, resulting in high cell viability. In the rat study, numerous CaOx crystals were found in the rats received EG only in 2 weeks, whereas PE significantly (~50%) reduced such EG-induced crystal deposits. Moreover, a ~1.2-fold increase in OXS with EG in the rat kidney was yet reduced by ~60% with PE, demonstrating antioxidant activity of PE.
Conclusions: The mushroom extract PE shows its antioxidant activity against H2O2-exerted OXS in MDCK cells. PE is also capable of preventing the CaOx crystal formation (~50%) in the rat kidneys, plausibly through its antioxidant activity. Therefore, PE could be a promising natural antioxidant, capable of protecting renal cells from OXS and potentially preventing the CaOx crystal formation.

Key words: antioxidant; calcium oxalate; mushroom extract; oxidative stress; rats.






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.