This research was constructed quantitatively and qualitatively on amoxicillin residual from the degradation process by the isolated soil fungi. Two amoxicillin-degrading fungi species were isolated from a dairy farm with global positioning system coordinate 2°59′28.8 ?N 101°44′00.5?E, where veterinary antibiotics have been used extensively. The isolated fungi were morphologically and molecularly identified as Aspergillus tamarii isolate 58. Lichtheimia ramosa strain R. Both fungi fermented at room temperature with 120 rpm at 96 hours, and later, amoxicillin residue was quantified. In High-performance liquid chromatography with photodiode-array detection analysis of the amoxicillin residue, A. tamarii isolate 58 degraded amoxicillin at a degradation rate of 49.0%, compared to 42.0% for L. ramosa strain R. This finding is positively supported by the antimicrobial assay of the amoxicillin residual against Escherichia coli ATCC 25922, Bacillus subtilis ATCC 19615, and Staphylococcus aureus ATCC 25923. Besides, the structure of the fungi before and after 1,000 ppm amoxicillin exposure at 0 and 96 hours was compared using a scanning electron microscope, which resulted in the observation of fewer shrinkage spores. In conclusion, the antibiotic amoxicillin is effectively degraded by both soil fungi, A. tamarii isolate 58 and L. ramosa strain R. Hence, the findings of this study provide a new alternative to degrade amoxicillin antibiotic pollution safely.
Key words: Amoxicillin, Aspergillus tamarii isolate 58 and Lichtheimia ramosa strain R
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