Background: The aim of current study to Isolation and identification of causal agent aerobic bacteria which Cause a variety of diseases in cows and Evolution of antimicrobial activity of aqueous, ethanolic extracts of against pathogenic bacteria isolate.
Material and Methods: A total 100 samples (Milk, Feaces, bone fracture, ocular discharge and mouth inflammation) were taken from Various diseases in cows. The results showed to the type and ratio of bacterial isolated were Staphylococcus aureus 35 (31.8 %), E coil 25(22.7%), proteus mirabilis 20 (18.8%), Klebsilla puenmonia 15 (13.6 %) staphylococcus epidermidis 10 (9.1%), and Micrococcus .Spp 5(4.5%). The antibiotic sensitivity results showed the Ciprofloxacin is the drugs of prefer use for treatment to a variety of diseases in cows.
Results: The results ethanolic extracts showed capability of Peganumharmala L to inhibition growth pathogenic bacteria. The greatest inhibition zone reached 55 mm to ward Klebsiella pneumonia at concentration 200%. while the range inhibition zone (45, 4, 35, 30, 27, 25 and 15) mm at concentrations (100%, 50%, 25%, 12.5%, 6.25%, 3.25% and 1.5%) respectively. and lowest inhibition zone zero mm toward proteusmarbilis at concentration 200%. while the range inhibition zone (30, 22, 24, 24, 20, 17 and 16) mm at concentration (100%, 50%, 25%, 12.5%, 6.25%, 3.25% and 1.5%) respectively.
While the results aqueous extract showed capacity of Peganumharmala L to inhibition growth pathogenic bacteria. The maximum inhibition zone reacher 50 mm to ward Klebsiella pneumonia at concentration 200%. And the range inhibition zone (49, 35, 30, zero, zero and zero) mm at concentration (100%, 50%, 25%, 12.5%, 6.25%, 3.25% and 1.5%) respectively while minimum inhibition zone zero mm were E. coil at concentration (200%, 100%, 50%, 25%, 12.5%, 6.25%, 3.25% and 1.5%) respectively.
Key words: Evaluations, antimicrobial, activity, aqueous, ethanolic extracts, Peganumharmala L., pathogenic,Various diseases, cows.
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