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Technical Note

EJMCM. 2019; 6(1): 14-19


Increased expression of oxidative phosphorylation genes in breast cancer cells

Jolanta Rzymowska, Brygida Slaska, Izabella Cisek, Katarzyna Wach, Sebastian Mazur, Paulina Stachyra, Ludmi& 322;a Grzybowska-Szatkowska.

Abstract
Aim: The aim of this study was to analyse the expression at the mRNA level of cytochromeb,
subunits COI, COII, COIII of complex IV, ATP6 and ATP8 and to perform aquantitative analysis
of the activity of ATP synthase as complex V of the respiratory chain, responsible for ATP
synthesis and break down of ATP to ADP in breast cancer. Methods and Results: The level
of gene expression at the mRNA level was evaluated on the basis of the light intensity of
fluorochrome or fluorescein in the cells. Determination of the activity and location of the ATP
synthase in cells was carried out using hybridization. In the formulations of breast cancer there
was a higher genes expression at the mRNA level for all the examined genes as compared
with non-cancerous tissue. The ATP activity was also higher in preparations obtained from
breast cancer cells compared with the control tissue. Conclusions: The results confirm
mtDNA incorporation to nDNA in neoplastic cells. They point to increased expression at mRNA
level for COI, COII, COIII, ATP6 and ATP8 in breast cancer cells compared with control tissue.
Increased amount of ATP synthase points to increased ATP in a neoplastic cell.

Key words: breast cancer, mitochondrial genes, gene expression, oxydative phosphorylation






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