Volvariella volvacea is an edible and nutraceutical tropical mushroom species. This study reports the optimal culture conditions for efficient growth of mycelia of the three wild strains of V. volvacea (La Clementina, Vinces, and Montalvo) from Ecuador. The mycelial growths were evaluated on the three culture media, pH, aeration, illumination, and temperature. Mycelia of the three strains grew best on Sabouraud dextrose agar at pH 6.57.5 when incubated in either sealed and unsealed conditions at 2628°C and 32°C. Vinces and Montalvo strains favored dark and lighted conditions, respectively, whereas La Clementina favored both. Therefore, the three exotic strains of V. volvacea could be commercially cultivated in the Philippines and utilized in various applications.
Key words: Ecuador strains of V. volvacea, optimum culture conditions, physical factors, Sabouraud dextrose agar.