This work carried out to analyze the microbiology and physicochemical parameters of water in some Nile Tilapia fish farms in Kafrelsheikh Governorate. Total bacterial and total Coliforms count were analyzed, as well as the presence of E.faecalis, E.coli, and E.coli O157 in both fish and farms water. The level of total bacterial count in water varied between 4.3x104 and 1.3x106 CFU/ml. The (MPN) of total Coliforms ranged between 1100/ml between different sampling sites. E.faecalis was verified in 28% and 38% of fish and water samples respectively E.coli was isolated in 18% and 34% from fish water samples. E.coli O157 verified in 2% of fish samples and does not present in water samples. Sero-grouping of E.coli isolates revealed that E.coli belongs to five serogroups (O6, O27, O125, O126, and O157). Results of microbiology were confirmed by results of physicochemical parameters of the water samples where values of PH, conductivity, salinity, dissolved oxygen, ammonia, nitrate, nitrite, sulfate, chlorine, chloride, total solids, total suspended solids, and total dissolved solids were significantly (P
Key words: Bacterial indicators, Chemical indicators, Fish farms, Water pollution, Kafrelsheikh Governorate.
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