Objective: To determine the prevalence of ESBL producing and carbapenem-resistant E. coli in UTI from a tertiary care Hospital.
Methodology: A total of 320 mid-stream urine samples were collected from patients suspected for UTI at the urology department of Jinnah postgraduate medical center, Karachi. Cystine lactose electrolyte deficient agar was used for culture. Antimicrobial susceptibility was determined by disc diffusion method. ESBL production was determined by double disc synergy test (DDST). The carbapenem resistance was determined by minimal inhibitory concentration method by Etest.
Results: Prevalence of UTI was higher (165/230, 71.7%) in age group of 21-40 years. The mean age of UTI patients was 38.75±15.01 years with dominancy of females (55.3%). Out of 320 samples, 256 (80%) were culture positive. E. coli was the most prevalent uropathogen 139(54.3%) followed by 59(23%), 24(9.4%), and 22(8.6%), Klebsiella, Proteus, and Pseudomonas species, respectively. E. coli showed highest resistance to ampicillin 87%, and lowest against meropenem (4.3%). Out of 139 E. coli, 92(66.2%) were ESBL producers, resistant to carbapenem was higher (5.03%) in ESBL producing E. coli in compassion to non- ESBL producers (1.43%).
Conclusion: The prevalence of ESBL producing E. coli was higher and carbapenem-resistant E. coli in urinary tract infections in community is still at lower prevalence.
Key words: UTI, carbapenem-resistant, E. coli.