Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

Taxonomic Validation of Areolate Grouper, Epinephelus areolatus (Perciformes: Serranidae) along the Nizampatnam Coast, India

Chatla Darwin, Padmavathi Pamulapati, Gatreddi Srinu.

Groupers (Perciformes: Serranidae: Epinephelinae) are the significant group of marine and estuarine fishes having ecological and commercial importance in tropical and subtropical waters. Identification of the genus Epinephelus is based on the color configuration and morphological characters. The homogeneity in the morphological characters has created confusion in the species identification of Epinephelus. Epinephelus areolatus has high economic value for its delicious taste, but its population has shown a notable decline recently due to overfishing. Due to morphological similarity, it was often confused with E. chlorostigma and E. bleekeri which leads to misidentification. Hence, correct identification is essential for the proper management of fishery resources. The present study was aimed to identify and validate E. areolatus using morphological and meristic characters including pyloric caeca count and pattern, vertebrae count through radiograph imaging, scale and otolith morphology, and DNA barcoding. Morphological and meristic characters of the species landed in Nizampatnam coast are in close proximity to those reported earlier as E. areolatus. Comparative molecular analysis was also carried along with other morphologically similar species viz., E. chlorostigma and E. bleekeri using mtCOI gene sequences. Mean inter-specific genetic distance revealed high similarity with E. bleekeri (0.109±0.012) and low similarity with E. chlorostigma (0.079±0.009). The Neighbor joining tree revealed distinct clades for three species with high bootstrap values. The partial DNA sequence of the Mitochondrial Cytochrome Oxidase I (COI) was established for the first time for E. areolatus from the Nizampatnam coast in the current study.

Key words: Epinephelus areolatus, DNA Barcoding, Morpho-meristic characters, Vertebrae, Pyloric caecum.

Similar Articles

Coenzyme-catalyzed electroinitiated reversible addition fragmentation chain transfer polymerization for ultrasensitive electrochemical DNA detection.
Ma N, Liu J, Liu B, Li L, Kong J, Zhang X
Talanta. 2022; 236(): 122840

SYNGR4 and PLEKHB1 deregulation in motor neurons of amyotrophic lateral sclerosis models: potential contributions to pathobiology.
Marques RF, Duncan KE
Neural regeneration research. 2022; 17(2): 266-270

The Cilioprotist Cytoskeleton , a Model for Understanding How Cell Architecture and Pattern Are Specified: Recent Discoveries from Ciliates and Comparable Model Systems.
Hufnagel LA
Methods in molecular biology (Clifton, N.J.). 2022; 2364(): 251-295

Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC) Using an Ă„KTA Start System.
Gotesman M, Wang Y, Madasu SC, Mitchell CA
Methods in molecular biology (Clifton, N.J.). 2022; 2364(): 237-249

Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans.
Fyfe JAM, Lavender CJ
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 63-69

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.

ScopeMed Web Sites