Reducing non-renewable material has raised significant concern for several years over the sustainable production of organic acids through bio-based methods in the world. One such way to overcome this problem is reactive extractant, in which appropriate extractants have been employed to recover various organic and inorganic acids. The extraction of acrylic acid by solvent extraction is an illustration of this technique.
The current study focuses on the synthesis of acrylic acid from acrylamidase produced by Bacillus tequilensis; succeeded by acid extraction from the amidase catalyzed reaction by solvent technique. Among various solvents ethyl acetate (2:1, v/v) was established as most appropriate solvents for the extraction. 65 mg of raw acrylic acid was recovered from 20 ml of amidase catalyzed reaction. Various analytical methods such as TLC, FTIR, and HPLC, MS were accomplished for the identification, validation and quantification of extracted acrylic acid. The m/z value of acrylic acid obtained in the extracted product was 73.18 which were similar to standard acrylic acid. From HPLC, almost 34 % bioconversion was quantified (3.4 mM) from 10 mM of acrylamide consumed. The extracted acrylic acid can be further exploited as chemical intermediates and pharmaceuticals in the future.
Key words: Acrylic acid; Acrylamide, Bacillus tequilensis; Reactive extractant; Acrylamidase; Biocatalysis.
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