Submerged fermentation of Pleurotus pulmonarius was carried out over a period of seven days in order to obtain phytotoxic metabolites. Separation of the metabolites was carried out using standard methods (mycelia, aqueous and organic extract). Antibacterial activity of the metabolites from the three extract were investigated against (Escherichia coli ST2747, Klebsiella pneumonia strain PMK1, Salmonella enterica sub specie entericaserevar typhimurium strain 08-1736 and Pseudomonas aeruginosa VRFPA04) using agar well diffusion technique. Phytochemical analysis was determined qualitatively, minimum inhibitory and minimum bactericidal concentration was determined by plate assays. Standard antibiotics were used to compare antibacterial activity of metabolites using Disc diffusion methods. The antimicrobial assay revealed that only the mycelial extract exhibited activity against the selected test strains. Phytochemical screening showed the presences of three phytoconstituents which includes, alkaloids, flavonoids saponins, tannin, steroids, glycosides, phenols and anthraquinones at varying concentration in the mycelia extract. The highest zone of inhibition of test organism was recorded in K. pneumoniae while the least was detected in P. aeruginosa. Phytotoxic metabolite obtained through submerged fermentation have been discovered to be antagonistic against pathogenic organisms which could be carried out to develop antimicrobial drugs to combat the menace of drug resistance as witness in some synthetic drugs.
Key words: phytochemicals, fungi, antimicrobial activity, agar well diffusion assay, mushroom,
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