Objectives: The aim of this study was to establish reference intervals according to laboratory data in a population and to assess the vitamin B12 (B12) and folate status related to reference intervals for all age and sex groups.
Materials and Methods: The results were obtained retrospectively from the laboratory information system of Balýkesir State Hospital. The 36284 patients (70.2 % for female, 29.8% for male) between 18- 80 ages were selected. The ages groups of the patient were separated into six subgroups (18–30, 31–40, 41–50, 51–60, 61–70 and 71-80 years). B12 and folate concentrations were measurement by ARCHITECT i2000sr (Abbott Diagnostics, Abbott Park, IL, USA) autoanalyzer. Extreme values were excluded by using IBM SPSS. The central %95 reference intervals were calculated using non-parametric method.
Results: The results of 20850 patients for B12 and 14183 for folate were evaluated. The ages mean of patients for B12 and folate were 48.9±16.3 and 49.7±16.6, respectively. Mean±SD concentrations of B12 and folate were 220±77 pmol/L and 13.9±6.5 nmol/L, respectively. 95% RIs were calculated to 97-397 pmol/L for vitamin B12 and 5.17-30.9 pmol/L for folate for all population. There are statistically significant different between female and male for B12 and folate. There is a significant difference between the age groups for folate, but is not a significant difference for B12 concentrations
Conclusions: In this study was found differences between the reference ranges recommended by the manufacturer and the reference ranges of our own population. Our results indicate that is important to determine the true reference range.
Vitamin B12, Folate, Reference range, Laboratory data, Turkiye
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