The aim of the study was to determine the antibiotic resistance profiles of Klebsiella pneumoniae, their extended-spectrum beta-lactamase (ESBL) production and biofilm formation on two different catheter surfaces along with its antibiotic resistance profile. Nearly, 47 K. pneumoniae strains were procured from K.A.P. Viswanatham Government Medical College, Trichy, Tamil Nadu, India. The tested strains exhibited 29 distinct antibiotic resistance patterns. From 47 K. pneumoniae, 93.6% (n = 44/47) were screened positive for ESBL by phenotypic confirmatory disk diffusion test (PCDDT). Furthermore, all the isolates were subjected to a double disk synergy test (DDST), and only 82.9% (n = 39/47) of isolates were found to be positive. However, in the modified double disk synergy test (MDDST) 97.8% (n = 46/47) isolates were positive, whereas in the direct modified three-dimensional test (DMTDT) 36.1% (n = 17/47) isolates were found to be positive and indirect modified three-dimensional test (IMTDT) it was 87.2% (n = 41/47). The quantification of K. pneumoniae biofilm formation over two distinct catheter surfaces revealed that polyvinyl chloride (PVC) based catheter was found to be adhered to a high number of cells than in silicon elastomer bonded catheter surface. Similarly, the biofilm formation of the strains grown with glucose in trypticase soya broth (TSBG) enhanced adherence ability. Moreover, enhanced antibiotic resistance of K. pneumoniae was found in the strains following the biofilm development. It is concluded that the existence of ESBL-positive isolates and glucose or other sugar substrates facilitates biofilm development, resulting in high resistance to several antibiotics. This circumstance is detrimental to human health.
Key words: K. pneumoniae, antibiotic resistance, ESBL, biofilm, catheters
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