Abstract

Background:
A good animal model was essential to understand the mechanism of NAFLD. It was needed to explore the NAFLD drug candidate target.

Aim:
This study aimed to determine the optimal fructose concentration and induction period for the NAFLD animal model.

Methods:
A total of 28 adult male Sprague Dawley rats were divided randomly into seven groups: standard diet (control, P1), three groups HFD-C with 30% w/v fructose for 4 weeks (P2), 6 weeks (P3), 8 weeks (P4), and three other groups HFD-C with 60% w/v fructose for 4 weeks (P5), 6 weeks (P6), and 8 weeks (P7). The blood sample and liver tissue were collected at the end of each period. We used spectrophotometry to measure the transaminase enzyme and metabolic parameter concentration. Liver tissue was evaluated by steatosis activity and fibrosis (SAF).

Results:
The steatosis of HFD-C with fructose groups (P2, P3, P4, P5, P6, and P7) were significantly higher than control group (P

Key words: Animal model; Fatty liver; NAFLD; Sprague Dawley.