A simple, precise and rapid reverse phase HPLC-PDA method has been developed and validated for the simultaneous analysis of hydroquinone (HYQ), Dexamethasone (DEX), triamcinolone acetonide (TSA), hydrocortisone acetate (HYA), betamethasone valerate (BEV) and retinoic acid (REA) in the cream dosage form. The mixture of HYQ, DEX, TSA, HYA, BEV and REA was separated on Waters X Bridge C18 5 μm column (4.6 mm × 250 mm). All separations were performed with a 2998 Photodiode Array (PDA) detector on 210-400 nm wavelength, 400C on column temperature and flow rate at 1,2 ml/minutes. The mobile phase was Acetonitrile (ACN): 0.1 % formic acid with gradient system. The retention time of HYQ, DEX, TSA, HYA, BEV and REA was found to be 3.697, 9.130, 9.470, 9.964, 14.919 and 21.202. The method showed linearity with correlation coefficient 0.9990, 0.9991, 0.9984, 0.9991, 0.9997 and 0.9991 over the range 0f 25-150 µg/ml for HYQ, DEX, TSA, HYA, BEV and REA respectively. The mean recoveries were found to be in the range of 99.05 100.96% for all component. This newly developed method can be used in routine analysis of HYQ, DEX, TSA, HYA, BEV and REA in the cream formula, whether for qualitative interest in cosmetic preparations or for quantitative interest in drug preparations.
Key words: Cream, High performance liquid chromatography, Hydroquinone, Dexamethasone Triamcinolone acetonide, Hydrocortisone acetate, Betamethasone valerate, Retinoic acidd.
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