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Original Article

Majmaah J Heal Sci. 2015; 3(2): 13-18


Prevalence and Antibiotic Resistance of Cronobacter Spp. Associated with Powdered Infant Milk Formulas and Dried Milk in Saudi Arabia

Khalid M Aljarallah.




Abstract

Introduction: This study examined the presence of the pathogen Cronobacter spp. in powdered infant milk formulas (PIMF), and dried whole and skim milk samples distributed in Saudi Arabia.

Methodology: Culture-based method, biochemical confirmation test and real-time PCR were used for detection.

Results: Cronobacter spp. was detected in 10% of PIMF and 20% of dried whole milk, but not in dried skim milk samples. Real-time PCR showed that PIMF samples generally contained higher numbers of Cronobacter spp. than dried whole milk samples. Isolates showed resistance to 9 of 10 antibiotics, being more frequently resistant to penicillin and tetracycline Both single and multidrug resistance was observed.

Conclusions: This study reports the association of antibiotic-resistant Cronobacter spp. with PIMF and dried whole milk products distributed in Saudi Arabia.

Key words: Cronobacter, antibiotic resistance, infant milk formulas, real-time PCR






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