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Original Article

JVS. 2018; 3(1): 1-9
doi: 286803


Molecular characterization of foot and mouth disease virus (O-EA3) isolated during 2016 outbreak in Egypt

Muhammad Gamal Khodary, Ayman Hanea El-Deeb, mohamed maged Emara, Othman El mahdy Othman, Hussein Ali Hussein.

Abstract
Summary:

Background/Aim:

In January 2016, several cases of clinical signs and mortalities suggested foot and mouth disease (FMD) outbreaks among cattle and buffalo herds in Egypt. This study aimed to investigate the contribution of Foot and Mouth Disease Virus (FMDV) and characterization of the circulating strains associated with the outbreaks.

Material and methods:

The collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular fluids were tested by real time Reverse transcriptase polymerase chain reaction (rRT-PCR) for detection of FMDV. The highly viral loaded FMDV positive samples were typed by conventional reverse transcriptase polymerase chain reaction (RT-PCR). Sequencing and phylogenetic analysis of 6 representative samples were performed.

Results:

Testing of 80 collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular fluids by real time Reverse transcriptase polymerase chain reaction (rRT-PCR) revealed 65 out of 80 FMDV positive samples (81%).Typing of the highly viral loaded FMDV positive samples (40 out of 65) by conventional reverse transcriptase polymerase chain reaction (RT-PCR) revealed that all tested samples belonged to serotype O of FMDV. Sequencing and phylogenetic analysis of 6 representative samples clustered the detected viruses with O topotype East Africa-3 (EA-3) viruses.

Conclusion:

The newly identified 2016 viruses clustered in distinct clade in the phylogenetic tree other than the serotype O viruses isolated in previous outbreaks in Egypt, indicating the likelihood of new incursions into Egypt. These strains were closely related to previously characterized strains circulating in Sudan and sub-Saharan Africa suggesting continued circulation of EA-3 topotype of FMDV in the region.

Key words: Foot and Mouth Disease Virus (FMDV), Serotype O, Topotype East Africa-3(EA-3), Polymerase chain reaction(PCR), Egypt


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