Home|Journals|Articles by Year|Audio Abstracts
 

Original Article

AJVS. 2018; 58(1): 102-108


Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk

El Sayed F.H. Ibrahim, Ashraf M. Abd El wahab, Samy A. Khalil, Helmy A. Torky.




Abstract

Bacterial resistance to antibiotics of Beta lactam group is increasing worldwide threatening the public healthcare due to the limited treatment substitutions. Beta lactamase especially Extended Spectrum Beta Lactamase (ESBL) enzymes are responsible for the most important resistance mechanisms of the Enterobacteriacae to antibiotics of Beta lactam group. In this study, in order to investigate the prevalence of extended-spectrum β-lactamase among Enterobacteriacae isolated from clinically mastitis and apparently normal cattle. A total number of 500 milk samples were collected from cow milk. A total number of 100 ESBL Enterobacteriaceae isolates were obtained. Bacterial isolates were identified by VITEK 2 compact and then ESBL enzymes were identified phenotypically. Estimation of sensitivity of these isolates against variety of antibiotics by Disk Diffusion Sensitivity Test was carried out. Finally ESBL enzymes were identified by Real Time Polymerase Chain Reaction (rPCR). The results revealed that, out of the 100 Enterobacteriacae, 17 isolates (17 %) were positive phenotypically for ESBL. Of which, 14 isolates were positive for genotypically for ESBL. Furthermore, it was found that CTX-M Gene is the most prevalent gene in the Enterobacteriacae isolates

Key words: Enterobacteriacae,antibiotic resistance ,






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.