Indwelling Medical Devices are significant in patient care and, critical patients easily become infect-able microbial contamination. The isolate and identify the bacterial biofilm that contaminated indwelling catheter tips for renal dialysis machines among patients and the associated resistance of antibiotics in Brack Hospital. One hundred of catheter tips were collected from patients. 2 inches of the distal tip of the catheter were clipped directly into a sterile broth. The culture was by rolling the tip across blood agar and incubated aerobically at 37C° for 18-24 hours. Bacterial isolates were Staphylococcus aureus 33%, Bacillus spp. 9.7%, Klebsiella. pneumoniae 8.3%, Escherichia. coli 8.3%, Staphylococcus albus 8.3%, Enterobacter aerogenes 8.3% Enterobacter spp. 6.9%, Proteus mirabilis 6.9%, Klebsiella ozaenae 6.9%, and Acinetobacter 2.7%. The resistance was 100% against Ampicillin. The 100% were resistant to Ceftazidime, Aztreonam but variable with the others and 100% sensitive to Imipenem with statistically significantly difference (P = 0.00).
Key words: Bacterial Biofilm, Catheter Tips, Renal Dialysis Machines, Brack, Libya, Hospital
|
