Helicobacter pylori infected gastric mucosa; the host immune response is unable to clear the infection and may contribute to the associated pathogenesis; CD74 expressed on the surface of gastric epithelial cells, as an adhesion molecules used by H. pylori that may contribute to the proinflammatory immune response seen during infection. This study aimed to investigate the role of local inflammatory cells infiltrating gastric mucosa that stained with IFN- and IL-6 monoclonal antibodies, as well as CD74 expressed on gastric epithelial cells in the immunopathogenic association with H. pylori gastritis. After the diagnosis of H. pylori infection by invasive and non-invasive diagnostic tests, patients were grouped as H. pylori positive, (n=47) and H. pylori negative (n=17). The immune staining of IL-6 and IFN- were positive at high level in 89.4% and 91.5%, respectively in H. pylori infected patients. In addition, the presence of specific stained lamina propria (MNCs and PMNs) cells with IL-6 and IFN- were significantly higher (P=0.0001) in the H. pylori-infected than in uninfected subjects; as well as a significant difference in the CD74 expression (p= 0.005) between infected and uninfected patients. It is concluded from this study that there was a significant difference between infected and uninfected biopsy specimens in the expression of CD74 in association with increase local presence of IFN- and IL-6, as well as PMNCs and MNCs, in the gastric mucosa.
Key words: Helicobacter pylori; CD74; Immunohistochemistry (IHC); interleukin-6 (IL-6); gamma interferon (IFN-ɣ); polymorphonuclear cells (PMNs); Mononuclear cells(MNCs)
|
