Home|Journals|Articles by Year|Audio Abstracts
 

Original Research

Egypt. J. Exp. Biol. (Zoo.). 2007; 3(0): 251-260


CYTOGENETICAL AND MOLECULAR STUDIES ON TWO COMMON SPECIES OF BATS FROM EGYPT, ROUSETTUS AEGYPTIACUS AND TAPHOZOUS NUDIVENTRIS

Wael Y. Attia, Nabila I. El-Desouki, Gamal A. Madkour, Abd El-Hady I. Sayed, Sobhy E. Hassab El-Nabi, Aml I. El-Refaiy.




Abstract

In the present study, the cytogenetic and genetic variations were examined to determine the genetic behavior and relationships between two common species of bats from Egypt, fruit-eating bat, Rousettus aegyptiacus and insectivores bat, Taphozous nudiventris. From the cytogenetic study, it was found that the chromosomal number of R. aegyptiacus is 36, including 17 pairs of autosomes and one pair of sex chromosomes. These chromosomes were divided into four groups according to their lengths. Group I ranges between (9.5-7.9 µm), including chromosome pairs number 1-4. Group II ranges between (6.3 - 5.5 µm), including chromosome pairs number 5-10. Group III ranges between (4.5 - 2.2 µm), including chromosomes number 11-17. Group IV including sex chromosome pair where X= 5 and Y= 0.9 µm. However, the chromosomal number of T. nudiventris is 42 in somatic cells, consisting of 20 pairs of autosomes and one pair of sex chromosomes. According to their relative length, they are divided into four size groups. Group I ranges between (9.5 - 8.3 µm), including chromosome pairs number 1-4. Group II ranges between (6.2 - 4.7 µm), including chromosome pairs number 5-11. Group III ranges between (3.6 - 2.4 µm), including chromosomes number 12-20. Group IV including a pair of sex chromosomes where X= 2.3 and Y= 1 µm. In the molecular study, the genetic variation and similarity coefficients were estimated using Random Amplified Polymorphic DNA (RAPD) finger printing. Five arbitrary primers (A1, A12, A3, A5, and A6) generated RAPD markers ranging from 194-1353 base pair. At primer A1, DNA of R. aegyptiacus appeared with four bands (281, 303, 577, and 843) while DNA of T. nudiventris appeared with only one band (287). At primer A12, DNA of R. aegyptiacus appeared at five bands (280, 290, 303, 339, and 815) while DNA of T. nudiventris appeared at four bands (279, 281, 310, and 919). At primer A3, DNA of R. aegyptiacus appeared at four bands at 280, 300, 603 and 713 while DNA of T. nudiventris appeared at three bands at 274, 278 and 281. At primer A5, DNA of R. aegyptiacus appeared at four bands at 540, 581, 1028 and 1242 while DNA of T. nudiventris appeared at six bands at 310, 472, 564, 752, 872, and 1174. At primer A6, DNA of R. aegyptiacus appeared at three bands at 432, 516 and 734. DNA of T. nudiventris appeared also at three bands at 452, 552, and 893. From these results, it can be concluded that: (1) The bands of DNA for each species is similar (280 and 281) only at primer A3 and A12 respectively. (2) By calculating the number of RAPD bands shared between the two species and the total numbers of bands for each species, the genetic similarity is 0.32 indicating low genetic similarity and large distance between them. (3) The variation of chromosomal and DNA analysis between the two species may be related to their ecological conditions and nutrition.

Key words: Chromosomes, Cytogenetic, Random Amplified Polymorphic DNA, bats






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.