Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Research

Egypt. J. Exp. Biol. (Bot.). 2011; 7(2): 261-267


EXPRESSION PROFILING OF ZYGOPHYLLUM COCCINEUM AND PEGANUM HARMALA UNDER SALT STRESS

Karam M. Anwar Amin S. Ali Abd El-Gawad M. El-Saied.

Abstract
For their sustainable utilization, seeds of three populations of each of Zygophyllum coccineum L. and Peganum harmala were collected and their response to salinity was evaluated through soil analysis, two days interval monitoring of seed germination, quantitative estimation of proteins and protein electrophoresis after treatment with 0, 50, 100, 150, 200, and 250 mM NaCl. Salinity reduced the percentage of germination, delayed the time required for germination, increased the amount of proteins. The germination percentage indicated that Z. coccineum collected from Wadi Hagool tolerates up to 150 mM, while P. harmala tolerated salinity up to 150 mM in Burg El-Arab and Siwa. As the severity of salinity increased, the amount of protein was reduced. The SDS-PAGE revealed specific bands in response to salinity. Populations collected from more saline soils were more tolerant to severe salinity treatment and were considered as valuable resources. Newly synthesized protein bands were observed under different concentration of NaCl at different time intervals. The time required for the synthesis of stress-related proteins has been estimated, offering a starting point for further analysis of the newly synthesized proteins e.g. their amino acid sequence, their corresponding mRNA and even their coding sequence.

Key words: Protein, Zygophyllum coccineum, Peganum harmala, salt stress, germination


Full-text options

Full-text Article




Advertisement
American Journal of Diagnostic Imaging

SUBMIT YOUR ARTICLE NOW




ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.