Objective: To evaluate biofilm formation, culture and sensitivity pattern after Double J stenting in patients with obstructive uropathy.
Methodology: This is descriptive cross sectional study was conducted at urology and pathology departments of SMBBMU Larkana and Microbiology institute SALU, Khairpur from January 1, to July 31, 2018. Double J stent was removed from the 55 patients by endoscopic instrumentation. Lower or upper part of DJ piece was cut from stent and washed with sterile saline to remove surface contaminant then transferred to tryptic soy broth for culture and sensitivity.
Results: Out of 55 patients, mean age in male was 27±13.1 and in female 22±13.8 years. Among these, 15(27.27%) patients were found biofilm formation which occured in patients in whom stent was kept for less than six weeks. Overall, biofilm formation was seen in 45(81.81%) patients. Male were more prone for biofilm formation; 30 out of 45 (66.66%), while 15(33.33%) female developed biofilm formation. From all patients, 22(40%) patients had positive Culture. The commonest organisms seen was E. coli, 15 out of 22 (68.18%), Pseudomonas 3(13.3%), Klebsilla 2(9.09%), Staph. coagulase ve 2(9.09%). Resistance pattern of antibiotics was seen as Quinolone (50%), Cephalosporin (60%), Gentamycin (55%), B-lactamase (30%) and Penicillin (65%). Sensitivity pattern showed as Amikacin (82%), Nitrofurantoin (92%), Fosfomycin (93%), piperacillin/Tazobactam (95%) and Meropenam (96%).
Conclusion: Our study showed that biofilm formation and positive culture was associated with time period of stenting. We recommend earlier removal of DJ stent and culture and sensitivity of stent to prevent uro-sepsis following stent removal.
Key words: Obstructive uropathy, Double J stent, biofilm formation, nephrolithiasis.
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