Abstract

Candidiasis and other fungal infections are becoming more frequent. The search for appropriate antibiotics for fungal infections is still ongoing. Pseudomonas azotoformans University of Indonesia Culture Collection (UICC) B-91 has potential as an anticandidal. The purpose of this study was to determine the mechanism of inhibition of the metabolite of P. azotoformans UICC B-91 from the medium against morphologically pathogenic fungi. The results showed metabolite compound P. azotoformans UICC B-91 at a concentration of 100 and 80 mg/ml had strong inhibitory power, and 60 mg/ml had moderate inhibition. Determination of the minimum inhibitory concentration value of the medium solution of P. azotoformans UICC B-91 against Candida albicans American Type Culture Collection (ATCC) 10231 was at a concentration of 7.50 mg/ml. Microscopic observation of C. albicans ATCC 10231 after the addition of P. azotoformans UICC B-91 medium under the light microscope showed inhibition of the transition from yeast to hyphae. Microscopic observation of C. albicans ATCC 10231 after the addition of P. azotoformans UICC B-91 medium under scanning electron microscope showed changes in the yeast cell surface become uneven. The mechanism inhibition of metabolites from P. azotoformans UICC B-91 medium against C. albicans ATCC 10231 is through diffusion that disrupts cell membrane function or inhibits transition from yeast to hyphal form.

Key words: anticandidal; C. albicans ATCC 10231; inhibition; mechanism; P. azotoformans UICC B-91