Fourier transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, especially mid-infrared, has fulfilled the need to counter the rising number of adulterated herbal products in society. The non-destructive, rapid, and inexpensive methods are criteria found in FTIR spectroscopy. This study aimed to develop a combination method between FTIRATR spectroscopy and chemometrics to analyze the quaternary model of adulterated herbal products claimed as analgesics. The samples consist of three types of analgesic herbal products (Jamu Pegal Linu, Jamu Encok, and Jamu Sakit Pinggang), prednisone, metamizole, diclofenac sodium, and quaternary mixtures were prepared and measured using FTIR spectrophotometer on absorbance mode at wavenumbers range 4,000–650 cm−1. Principal Component Analysis (PCA), Partial Least Square Regression (PLSR), Principal Component Regression (PCR), and Discriminant Analysis were applied for analyzing spectra. The PCA result showed good differentiation between samples. The output of multivariate calibrations was excellent in line with statistical parameter value. The accuracy and precision from PLSR and PCR of quaternary mixtures were shown by a low value of root mean square error of prediction and root mean square error of calibration and R2> 0.99. Therefore, we suggested the combination method between FTIR-ATR and chemometrics as screening analytical technique to detect any adulterant drugs in herbal products.
Key words: Jamu, Mid-Infrared, Multivariate Analysis, Counterfeit, Analgesic Drugs
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